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PI80196

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  • PI80196
  • Pierce ECL 2 Western Blotting Substrate, 100mL kit

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.

Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.

Includes: Detection Reagent 1 and 2
Highlights:

  • High sensitivity — detect targets down to the low-picogram level
  • Long signal duration — sustained light output for as long as five hours
  • More imaging options — X-ray, CCD or laser-based imagers
  • More affordable — high quality and performance at a lower price than other suppliers' similar substrates

View Specifications

Images for Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate


  • PI80196
  • Pierce ECL 2 Western Blotting Substrate, 100mL kit
PI80196

PI80196


(3 item(s) in product Group)
  • Items
  • Specifications
Catalog No. Description Price per Unit Quantity & Availability Add Item(s)
PI80197
Thermo Scientific
No.:80197
For: 250cm2 of membrane
Each for $90.78

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PI80196
Thermo Scientific
No.:80196
For: 10 mini-gel blots; 1000cm2 of membrane
Each for $316.68

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PI80196X3
Thermo Scientific
No.:80196X3
For: 30 mini-gel blots; 3000cm2 of membrane
Each for $644.28

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Description

Highlights:

  • High sensitivity — detect targets down to the low-picogram level
  • Long signal duration — sustained light output for as long as five hours
  • More imaging options — X-ray, CCD or laser-based imagers
  • More affordable — high quality and performance at a lower price than other suppliers' similar substrates



Recommended Storage: Store from 2° to 8°C.