Electrophoresis, Western Blotting and ELISA

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Thermo Scientific™ PageRuler™ Plus Prestained 10-250kDa Protein Ladder Available on GSA/VA Contract for Federal Government customers only.

Mixture of 9 blue-, orange- and green-stained proteins (10 to 250kDa) used as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ Pierce™ High Binding Streptavidin Coated Plates Available on GSA/VA Contract for Federal Government customers only.

Bind biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available streptavidin-coated plates.

Thermo Scientific™ Pierce™ TMB Substrates Available on GSA/VA Contract for Federal Government customers only.

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer Available on GSA/VA Contract for Federal Government customers only.

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Thermo Scientific™ Pierce™ Silver Stain Kit Available on GSA/VA Contract for Federal Government customers only.

Stain proteins in 1D and 2D gels with this rapid, ultra-sensitive and versatile silver stain system that yields consistent and reliable results.

Alfa Aesar™ Mouse Osteocalcin Enzyme Immunoassay Kit

For measuring levels of osteocalcin in mouse serum and plasma samples

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate Available on GSA/VA Contract for Federal Government customers only.

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Pierce™ Streptavidin Poly-HRP Conjugate Available on GSA/VA Contract for Federal Government customers only.

Amplify signal and improve detection of biotinylated antibodies for IHC and other methods using this streptavidin conjugated with HRP polymers.

Thermo Scientific™ Pierce™ Maleimide-Activated HRP Conjugation Kit

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Thermo Scientific™ Pierce™ Human MIP-1 alpha ELISA Kits

Measure levels of human macrophage inflammatory protein-1 alpha (MIP1a) in human serum, plasma and culture supernatant samples using 96-well plates.

Thermo Scientific™ Restore™ Western Blot Stripping Buffer Available on GSA/VA Contract for Federal Government customers only.

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Thermo Scientific™ Pierce™ Nickel Coated Plates Available on GSA/VA Contract for Federal Government customers only.

Bind His-tagged fusion proteins for ELISA and other plate-based assays to study interactions of expressed recombinant proteins.

Thermo Scientific™ Pierce™ Recombinant Protein L and Conjugates Available on GSA/VA Contract for Federal Government customers only.

Detect mouse and human antibodies containing kappa light chains in immunoassays using this purified, biotinylated or peroxidase-conjugated, recombinant Protein L.

Thermo Scientific™ Pierce™ Fast Western Blot Kits, ECL

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

Thermo Scientific™ Pierce™ 96-Well Microplates Available on GSA/VA Contract for Federal Government customers only.

Bind antibodies with high capacity using these specially formulated polystyrene plates for ELISA and other immunoassays.

Thermo Scientific™ Pierce™ Streptavidin HRP Conjugate, High Sensitivity Available on GSA/VA Contract for Federal Government customers only.

Amplify signal in Western blot, ELISA or other biotin-based detection methods with this exclusive, high signal-to-noise, peroxidase-conjugated, ready-to-use streptavidin.

Thermo Scientific™ Pierce™ Silver Stain Kit for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Reversibly stain proteins in polyacrylamide gels to identify bands to excise; then destain the gel pieces for analysis by mass spectrometry.

Thermo Scientific™ UltraVision™ Quanto Detection System HRP

Deliver higher lab profitability and better staining by selecting the best antibody for each test with the Thermo Scientific™ UltraVision™ Quanto HRP DAB.

Thermo Scientific™ Pierce™ Lane Marker Sample Buffers Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker.

Thermo Scientific™ CL-XPosure™ Film (X-Ray Film) Available on GSA/VA Contract for Federal Government customers only.

Capture results of ECL-based Western blots and other chemiluminescent protein or nucleic acid assays using this economically priced, clear-blue X-ray film.

Invitrogen™ Avidin Protein and Conjugates

Perform Western blot, ELISA or other biotin-based detection methods using these purified and HRP-, AP- or FITC-labeled avidin molecules.

Thermo Scientific™ Blocker™ Casein Blocking Buffers Available on GSA/VA Contract for Federal Government customers only.

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit Available on GSA/VA Contract for Federal Government customers only.

Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.

Thermo Scientific™ Pierce™ Recombinant Protein A/G and Conjugates Available on GSA/VA Contract for Federal Government customers only.

Detect antibodies, especially IgG isotypes, from many different species hosts with this purified, alkaline phosphatase- or peroxidase-conjugated recombinant Protein A/G.

Thermo Scientific™ Pierce™ High Binding NeutrAvidin™ Coated Plates

Attain higher biotin-binding efficiency with these pre-blocked, ready-to-use high-capacity NeutrAvidin protein-coated microplates.

Thermo Scientific™ Pierce™ Stop Solution for TMB Available on GSA/VA Contract for Federal Government customers only.

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

Thermo Scientific™ Pierce™ Krypton™ Fluorescent Protein Stain

Stain proteins in polyacrylamide gels or transfer membranes for fluorescent detection with popular fluorescence imagers.

Thermo Scientific™ SuperSignal™ West Dura Chemiluminescent Substrate Available on GSA/VA Contract for Federal Government customers only.

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Thermo Scientific™ Clean-Blot IP Detection Reagents and Kit

Avoid interference from denatured IP antibody fragments in Western blots of immunoprecipitated proteins with these HRP and AP conjugates.

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