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Thermo Scientific™ 1-Step Heavy Protein IVT Kit

Express stable isotope-labeled (i.e., heavy) proteins by in vitro translation (IVT) for mass spectrometry using these cell-free expression kits.

$302.00


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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
PI88330
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Thermo Scientific™
88330
8 rxn kit Each for $302.00
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PI88331
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Thermo Scientific™
88331
40 rxn kit This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

The Thermo Scientific™ 1-Step Heavy Protein In Vitro Protein Expression Kits enable rapid cell-free expression of recombinant proteins containing stable isotope-labeled (i.e., heavy) amino acids.

The 1-Step Heavy Protein IVT Kit uses a unique HeLa cell lysate supplemented with heavy amino acids for in vitro translation (IVT) of proteins with 90 to 95% isotope incorporation efficiency in less than 8 hours. Heavy proteins expressed using this system can be used as mass spectrometry controls for sample prep loss, digestion efficiency determination or as quantification standards.

Highlights:

  • Efficient – express heavy proteins with 90-95% stable isotope incorporation

  • Functional – uses the human translational machinery to express more biologically active proteins than other IVT systems

  • Flexible – express light proteins or heavy proteins containing 13C615N2 L-lysine and/or 13C615N4 L-arginine

  • Convenient – perform transcription and translation in a single step

  • High performance – greater yields compared to rabbit reticulocyte in vitro translation

Recommended for:

Identify ideal peptides for targeted quantitation; Verify protein digestion efficiency; Control for protein sample prep variability and affinity enrichment loss

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