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R&D Systems™ 10X Spheroid Formation Matrix and Plate

Uses the addition of ECM proteins to non-spheroid forming cells to induce spontaneous spheroid formation. R&D Systems™ 10X Spheroid Formation Matrix and Plate optomizes this prossess, providing the necessary reagents to evaluate cells using this method.

Manufacturer:  R&D Systems™ 3500096SP

Catalog No. 35-000-96SP


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Description

Description

  • Dispense into working aliquots if needed
  • Repeated freeze-thaws will destroy product integrity
  • Simply harvest cells, resuspend in spheroid formation ECM, and then culture in a 96 well spheroid formation plate
  • The addition of ECM proteins to non-spheroid forming cells induced spontaneous spheroid formation, making the spheroid format compatible with most solid cancer cell models
  • Cell number and culture time determines spheroid size, and since each well produces one spheroid, researchers have complete control over spheroid dimensions with virtually no well to well variability
  • Recommend spheroids between 400 to 500μm in diameter
  • Once formed, these multicellular tumor cell aggregates can be treated with pharmacological compounds to evaluate the effect on tumor spheroid growth
    • Specific genes or pathways may be manipulated to evaluate their effect on expansion of the in vitro tumor
Specifications

Specifications

Product is stable for a minimum of 3 months from date of shipment. Store at -80° C; keep frozen. Dispense into working aliquots if needed; repeated freeze-thaws will destroy product integrity.
96 Tests
Stable for a minimum of 3 months from date of shipment
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Safety and Handling

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