CD290 (TLR10) Monoclonal Antibody (3C10C5), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 12290942

Catalog No. 12-290-942

Description

Description: This 3C10C5 monoclonal antibody reacts with human CD290, which is also known as Toll-like receptor 10 (TLR10). This type I transmembrane glycoprotein is expressed on B cells, monocytes, regulatory T cells (Tregs), and plasmacytoid dendritic cells. Moreover, CD290 expression is dependent on Foxp3 and NFAT in Tregs. Studies demonstrate that CD290 can self-dimerize, as well as heterodimerize with other members of the TLR family such as TLR1 and TLR2. Ligand binding results in association of the receptor with the adapter molecule MyD88 and subsequent activation of signaling pathways. Although the ligands of CD290 remain undefined, this receptor has been associated with the immune response against microbial and fungal infection and autoimmune diseases such as Crohn's disease and systemic lupus erythematosous. Applications Reported: This 3C10C5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3C10C5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Its exact function is not known. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Specifications

• Antigen: CD290 (TLR10)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9BXR5
• Gene Symbols: TLR10
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 81793
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 3C10C5
• Conjugate: PE
• Gene: TLR10
• Gene Alias: CD290; MGC104967; MGC126398; MGC126399; TLR10; toll like receptor 10; toll-like receptor 10; UNQ315/PRO358
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ