Phospho-PLCG2 (Tyr759) Monoclonal Antibody (4NPRN4), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 12986642

Catalog No. 12-986-642

Description

Applications Reported: This 4NPRN4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4NPRN4 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gamma1, PLC gamma2, PLC delta1, PLC delta2 and PLCe. PLC delta exists as four different isoforms. PLC delta1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC delta1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and alpha1B-adrenoreceptor signaling. Mouse PLC delta1 is highly expressed in brain, heart, lung and testis. PLC delta is abnormally accumulated in autopsied brains with Alzheimer's disease (AD), suggesting that it may play a role in the pathology of AD. PLC delta2 is markedly expressed in type II intestinal metaplasia and in the adenocarcinoma. When PLC delta2 is expressed in type I intestinal metaplasia, the metaplasia is generally considered benignant, yet evolves toward neoplastic transformation. Thus, PLC delta2 expression may be a possible marker of gastric malignant transformation.

Specifications

• Antigen: Phospho-PLCG2 (Tyr759)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P16885, Q8CIH5
• Gene Symbols: Plcg2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 234779, 5336
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 4NPRN4
• Conjugate: PE
• Gene: Plcg2
• Gene Alias: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2; APLAID; FCAS3; phosphoinositide phospholipase C; phosphoinositide phospholipase C-gamma-2; phospholipase C gamma 2; phospholipase C, gamma 2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C-gamma-2; Phospholipase C-IV; PLC gamma 2; PLC gamma2; PLC- gamma2; PLC gamma-2; PLCG2; Plcg-2; PLC-gamma; PLCgamma2; PLC-gamma-2; PLC-IV
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1 κ