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GE Healthcare 2D Protein Extraction Buffer-V

Designed to produce better spot resolution for 2D gel analysis. GE Healthcare 2D Protein Extraction Buffer-V offers a convenient way to prepare high-quality protein lysates. Suitable for use with 2D or 1D electrophoresis, liquid chromatography, protein expression and mass spectrometry applications.

Manufacturer:  GE Healthcare 28943527

Catalog No. 45-002-415

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.



Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. 2D Protein Extraction Buffer-V offers a convenient way to prepare high-quality protein lysates. This buffer is a modification of well-studied protein solubilization and IPG-strip rehydration buffers. It is designed to produce high spot resolution for 2D gel analysis.
  • Buffer is available as a dry powder, which is rehydrated with the provided diluent
  • Contains urea, thiourea and CHAPS with Diluent-II
  • Compatible with CyDye™ DIGE Fluors
  • Extraction Buffer-V is suitable for stronger solubilization effects


For 50mL sample solution
This study presents a comparison between different protein extraction buffers to investigate the extraction capabilities and differences between the buffers
Rat liver proteins were extracted using 2-D Protein Extraction Buffer-I to -VI, as well as a standard 2-D lysis reference buffer
Contains Urea, Thiourea and CHAPS
30mL diluent supplied with the dry buffer component
Instructions supplied with the buffer
2-D Protein Extraction Buffers at 15°C to 30°C
DILUENTS at 2°C to 8°C
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