BioVision Inc 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit: 2-Phosphoglycerate (2PG) Colorimetric/Fluorometric Assay Kit: The only kit on the market to measure 2PG. Measures 2PG levels in tissues & cells. Detection limit: less than 20 pmol. 100 assays.
Manufacturer: BioVision Inc K778100
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research. BioVision’s 2-Phosphoglycerate Assay kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types. BioVision charges an Ice pack fee for each order.
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