R&D Systems™ 3-D Culture Cell Loading Buffers
R&D Systems™ 3-D Culture Cell Loading Buffers provide an optimized and standardized solution for the isolation and normalization of cell lysates from 3-D Culture Matrix™ BME or Laminin I for subsequent biochemical analysis.
Manufacturer: R&D Systems™ 3448020CL
- Do not use past expiration date
- 3D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extra-cellular proteins comprising the hydrogel.
- Dilute 1:10 in cell lysates for western blotting
- 125mM Tris, pH 6.8, 4% SDS, 10% Glycerol, 0.1% Bromophenol Blue
|Store the unopened product at room temperature|
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