3-Nitrotyrosine Monoclonal Antibody (2A12), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA112770

Catalog No. 01-674-053

Description

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.

Specifications

• Antigen: 3-Nitrotyrosine
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 3mM sodium azide; pH 7.5
• Host Species: Mouse
• Purification Method: Ion-exchange chromatography
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: 2A12
• Conjugate: Unconjugated
• Gene Alias: 3-Nitro-tyrosine; C9H10N2O5
• Immunogen: 3-Nitrotyrosine-KLH.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Chemical