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ACROBiosystems Recombinant Protein;Human B7-1 / CD80 Protein, Fc Tag (HPLC-verified);HEK293;100UG;B71,CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
Supplier: ACROBiosystems B71H5259100UG
Recombinant Protein;Human B7-1 / CD80 Protein, Fc Tag (HPLC-verified);100UG;Human B7-1, Fc Tag (B71-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # NP_005182.1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4 (Cat. No. CT4-H82E4) with a linear range of 50-500 ng/mL (Routinely tested).;Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL ( 100 μl/well ) can bind Human CTLA-4, Strep Tag (Cat. No. CT4-H5284) with a linear range of 1-20 ng/mL (QC tested).;FACS assay shows that Human B7-1, Fc Tag (HPLC-verified)(Cat. No. B71-H5259) can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-1 is 1 μg/ml (Routinely
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