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ACROBiosystems Recombinant Protein;Human CTLA-4 / CD152 Protein;HEK293;100UG;CT4,CTLA4,CD152,CELIAC3,CTLA-4,GRD4,GSE,ICOS,IDDM12
Supplier: ACROBiosystems CT4H5229100UG
Recombinant Protein;Human CTLA-4 / CD152 Protein;100UG;Human CTLA-4, His Tag (CT4-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # NP_005205.2).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.4 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.;Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 1-6.4 ng/mL (Routinely tested).;Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with a linear range of 0.16-2.56 ng/mL (QC tested).;Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CTLA-4, His Tag (Cat. No. CT4-H5229) with an affinity constant of 25.7 nM as determined in a SPR assay (Biacore T200)
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