ACROBiosystems Recombinant Protein;FITC-Labeled Human Siglec-2 / CD22 Protein;HEK293;25UG;SI2,CD22,SIGLEC2,BL-CAM,SIGLEC-2,Siglec2,Siglec-2,SIGLEC2FLJ22814,T-cell surface antigen Leu-14

Supplier:  ACROBiosystems SI2HF2H625UG

Recombinant Protein;FITC-Labeled Human Siglec-2 / CD22 Protein;25UG;FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) is expressed from human HEK293 cells. It contains AA Asp 20 - Arg 687 (Accession # P20273-1). It is the FITC labeled form of Human Siglec-2 (Cat. No. CD2-H52H8).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 77.0 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human CD22 MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) with a linear range of 0.625-10 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).;293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Siglec-2 / CD22 Protein (Cat. No. SI2-HF2H6, 10 µg/ml) and C. FITC-labeled Protein Control. A. Non-transfected 293 cells and C. FITC-labeled Protein Control were used as negative control. RFP was u

Catalog No. 50-165-7581