ACROBiosystems Recombinant Protein;Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein;HEK293;25UG;IT1,Integrin alpha 4 beta 1,ITGA4&ITGB1,ITGA4&B1
Manufacturer: ACROBiosystems IT1H82W125UG
Recombinant Protein;Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein;25UG;Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (IT1-H82W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).;Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) & 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus.The reducing (R) protein migrates as 100-150 kDa (ITGA4) and 65-85 kDa (ITGB1) respectively due to glycosylation.;Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) with a linear range of 2-31 ng/mL (QC tested).
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