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ACROBiosystems Recombinant Protein;Biotinylated Human Siglec-2 / CD22 Protein, Fc Tag, Avi Tag (Avitag™);HEK293;25UG;SI2,CD22,SIGLEC2,BL-CAM,SIGLEC-2,Siglec2,Siglec-2,SIGLEC2FLJ22814,T-cell surface antigen Leu-14
Manufacturer: ACROBiosystems SI2H82F825UG
Recombinant Protein;Biotinylated Human Siglec-2 / CD22 Protein, Fc Tag, Avi Tag (Avitag™);25UG;Biotinylated Human Siglec-2, Fc Tag, Avi Tag (SI2-H82F8) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687 (Accession # P20273-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 103.9 kDa. The protein migrates as 115-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human CD22 MAb at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-2, Fc Tag, Avi Tag (Cat. No. SI2-H82F8) with a linear range of 1-16 ng/mL (QC tested).;293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Siglec-2 / CD22 Protein, Fc Tag, Avi Tag (Cat. No. SI2-H82F8, 10 μg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Pro
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