ACROBiosystems Recombinant Protein;Biotinylated Human PD-L1 / B7-H1 Protein, Fc Tag, Avi Tag (Avitag™);HEK293;25UG;PDL,PDL1,PD-L1,CD274,B7-H1,B7H1,B7-H,B7H,PDCD1L1,PDCD1LG1

Supplier:  ACROBiosystems PDLH82F225UG

Recombinant Protein;Biotinylated Human PD-L1 / B7-H1 Protein, Fc Tag, Avi Tag (Avitag™);25UG;Biotinylated Human PD-L1, Fc Tag (PDL-H82F2) is expressed from human 293 cells (HEK293). It contains AA Phe 19- Arg 238 (Accession # Q9NZQ7-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 53.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human PD-1, Fc Tag (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc Tag (Cat. No. PDL-H82F2) with a linear range of 10-313 ng/mL (QC tested).;Biotinylated Human PD-L1（Cat. No. PDL-H82F2）at 1 μg/mL (5 μL/well) can bind Human PD-1 (Cat. No.  PD1-H5221) with a linear range of  0.04-1.25 μg/mL(Routinely tested).;AlphaLISA shows that the binding of Biotinylated Human PD-L1（Cat. No. PDL-H82F2）to Human PD-1 (Cat. No.  PD1-H5221) was inhibited by increasing concentration

Catalog No. 50-165-8215