ACROBiosystems Recombinant Protein;Human PD-L2 / B7-DC Protein, Fc Tag (HPLC-verified);HEK293;100UG;PD2,PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2,PDL-2,B7DC

Supplier:  ACROBiosystems PD2H5251100UG

Recombinant Protein;Human PD-L2 / B7-DC Protein, Fc Tag (HPLC-verified);100UG;Human PD-L2, Fc Tag (PD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 200 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag (Cat. No. PD2-H5251) with a linear range of 0.156-0.625 μg/mL (QC tested).;Flow Cytometry assay shows that recombinant Human PD-L2, Fc Tag (Cat. No. PD2-H5251) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 1 μg/mL (Routinely tested).;Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L2, Fc Tag (HPLC-verified) (Cat. No. PD2-H5251) with an EC50 of 0.018 μg/ml (Routinely tested).

Catalog No. 50-165-8524