ACROBiosystems Recombinant Protein;Human PD-L2 / B7-DC Protein, mouse IgG1 Fc Tag;HEK293;100UG;PD2,PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2,PDL-2,B7DC
Supplier: ACROBiosystems PD2H52A5100UG
Recombinant Protein;Human PD-L2 / B7-DC Protein, mouse IgG1 Fc Tag;100UG;Human PD-L2, Mouse IgG1 Fc Tag (PD2-H52A5) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).;This protein carreis a mouse IgG1 Fc tag at the C-terminus, and has a calculated MW of 48.9 kDa. The predicted N-terminus is Leu 20. The reducing (R) protein migrates as 60-70 kDa in SDS-PAGE due to glycosylation.;Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag (Cat. No. PD2-H52A5) with a linear range of 10-156 ng/mL (QC tested).;Flow Cytometry assay shows that recombinant Human PD-L2, mouse IgG1 Fc Tag (Cat.No. PD2-H52A5) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L2 used is 0.1 μg/ml (Routinely tested).;FACS analysis shows that the binding of Human PD-L2, mouse IgG1 Fc Tag to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing
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