ACROBiosystems ACROBiosystems Biotinylated Anti-Cetuximab Antibodies (recommended for PK/PD)
Supplier: ACROBiosystems CEBBY3125UG
Antibody;25UG;IgG1/kappa;Recognizes Cetuximab specifically, no cross reactivity with other humanized antibodies.;Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Cetuximab F(ab')2.; ;Detection of cetuximab by bridging ELISA in serum. Immobilized Anti-Cetuximab Antibodies (Cat. No. CEB-Y29) at 2 μg/mL, add increasing concentrations of cetuximab (10% human serum) and then add Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 6 ng/mL. ;Immobilized cetuximab at 0.1 μg/mL (100 μL/well) can bind Biotinylated Anti-Cetuximab Antibodies (Cat. No. CEB-BY31) with a linear range of 0.39-25 ng/mL.
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