ACROBiosystems ACROBiosystems Biotinylated Anti-Adalimumab Antibodies (recommended for PK/PD)
Manufacturer: ACROBiosystems ADBBY1725UG
Antibody;25UG;IgG1/kappa;Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.;Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Adalimumab F(ab')2.; ;Detection of adalimumab by bridging ELISA in serum. Immobilized Anti-Adalimumab Antibodies (Cat. No. ADB-Y23) at 2 μg/mL, add increasing concentrations of adalimumab (10% human serum) and then add Biotinylated Anti-Adalimumab Antibodies (Cat. No. ADB-BY17) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 4.8 ng/mL. ;Immobilized adalimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Adalimumab Antibodies (Cat. No. ADB-BY17) with a linear range of 0.78-25 ng/mL.
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