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ACROBiosystems ACROBiosystems Anti-Rituximab Antibodies (recommended for ADA assay)
Manufacturer: ACROBiosystems RIBY36100UG
Antibody;100UG;IgG1/kappa;Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.;Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Rituximab F(ab')2.; ;Anti-Rituximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized rituximab at 1 µg/mL, add increasing concentrations of Anti-Rituximab Antibodies (Cat. No. RIB-Y36, 10% human serum) and then add biotinylated rituximab at 2 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 9.7 ng/mL.;Demonstration of the specificity of Anti-Rituximab Antibodies (Cat. No. RIB-Y36) to the rituximab.;Anti-Rituximab Antibodies (mouse IgG1, Cat. No. RIB-Y36) captured on CM5 chip via anti-mouse antibodies surface, can bind human rituximab with an affinity constant of 0.01 nM. ;FACS analysis shows that the binding of rituximab to 293F overexpressing
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