ACROBiosystems ACROBiosystems RFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag
Manufacturer: ACROBiosystems MSNHR2P425UG
Recombinant Protein;25UG;RFP Fusion Human Mesothelin (296-580), His Tag (MSN-HR2P4) is expressed from human 293 cells (HEK293). It contains AA Glu 296-Gly 580 (Accession # AAH09272.1).;This protein carries a RFP tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Anti-Human MSLN Mab, Human IgG1 at 10 μg/mL (100 μL/well) can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with a linear range of 0.078-0.625 μg/mL (QC tested).;can bind Human MS4A1 / CD20 Full Length Protein, His Tag (Cat. No. CD0-H55Ha)with an affinity constant of 1.21 nM as determined in a SPR assay (Biacore T200) (Routinely tested).;2e5 of the MSLN-CAR-293 cells were stained with 100 μL of 3 μg/mL of RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4). Non-transfected 293 cells were used as negative control (QC tested).
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