ACROBiosystems ACROBiosystems Human PDGF-BB Protein, Tag Free
Supplier: ACROBiosystems PDBH411220UG
Recombinant Protein;20UG;Human PDGF-BB, Tag Free (PDB-H4112) is expressed from E.coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).;Human PDGF-BB, Tag Free is a disulfide-linked homodimeric polypeptide chain containing 2×109 amino acids, and has a calculated MW of 12.4 kDa. The reducing (R) protein migrates as a 15 kDa polypeptide and the non-reducing (NR) disulfide-linked homodimer migrates as a 28 kDa protein.;Immobilized Human PDGF-BB, Tag Free (Cat. No. PDB-H4112) at 5 μg/mL (100 μL/well) can bind Human PDGFRB, Fc Tag (Cat. No. PDB-H5252) with a linear range of 0.039-0.625 μg/mL (QC tested).;Immobilized Human PDGF-BB, Tag Free (Cat. No. PDB-H4112) at 2 μg/mL (100 μL/well) can bind Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) with a linear range of 0.02-0.625 μg/mL (Routinely tested).;The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (Routinely tested).
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