ACROBiosystems ACROBiosystems Human EGF R Protein, His Tag, low endotoxin
Manufacturer: ACROBiosystems EGRH522A100UG
Recombinant Protein;100UG;Human EGF R, His Tag, low endotoxin (EGR-H522a) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag, low endotoxin (Cat. No. EGR-H522a) with a linear range of 0.8-6 ng/mL (QC tested).;Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Human EGF R, His Tag, low endotoxin (Cat.No. EGR-H522a) with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) ( Routinely tested).
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