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ACROBiosystems ACROBiosystems Human CD47 Protein, Mouse IgG2a Fc Tag, low endotoxin
Supplier: ACROBiosystems CD7H52A5100UG

Recombinant Protein;100UG;Human CD47, Mouse IgG2a Fc Tag, low endotoxin (CD7-H52A5) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).;This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.8 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 100-130 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) at 2 μg/mL (100 μL/well) can bind Human CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H52A5) with a linear range of 0.5-8 ng/mL (QC tested).;FACS assay shows that Human CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H52A5) can bind to ACHN cell expressing human SIRP-a. The concentration of CD47 used is 10 μg/mL (Routinely tested).
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