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ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD (E484K), His Tag (MALS verified)
Manufacturer: ACROBiosystems SRDC52H3100UG
Recombinant Protein;100UG;SARS-CoV-2 S protein RBD (E484K), His Tag (SRD-C52H3) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (E484K)). E484K mutaion was identified in the SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa.;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (E484K), His Tag (Cat. No. SRD-C52H3) with a linear range of 0.01-0.156 μg/mL (QC tested).;Serial dilutions of Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) were added into SARS-CoV-2 S protein RBD (E484K), His Tag (Cat. No. SRD-C52H3): Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) binding reactions. The half maximal inhibitory c
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