GE Healthcare HiTrap MabSelect SuRe Prepacked Columns
Use these columns in small-scale MAb purification in a continuous bioprocess. GE Healthcare Life Sciences HiTrap MabSelect SuRe (Superior Resistance) Prepacked Columns for fast purification of monoclonal antibodies (Mabs) from large sample volumes
Manufacturer: GE Healthcare 11003493
Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs. MabSelect media are suited for the high flowrates and pressures required when scaling up.
- Oriented coupling of recombinant Protein A ligand to the agarose matrix via an engineered C-terminal cysteine increases IgG binding capacity over other protein A media
- High capacity, low ligand leakage, and high-flow base matrix allow purification of Mabs at process scale
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification
- Designed for use with a syringe, a peristaltic pump, or a chromatography system
- Allows at least five times higher flowrates than conventional cross-linked agarose of similar porosity
- Recombinant Protein A ligand promotes specificity of binding to Fc region of IgG; permits one-step purification
- Same recombinant protein A ligand as MabSelect, but with a smaller particle size and greater porosity, for increased dynamic binding capacity
- Allows binding from samples with high expression levels of the monoclonal antibody
- Lowers overall production cost due to the possibility of processing concentrated feedstocks in fewer batches
- Akali-tolerant protein A-derived ligand allows the use of up to 0.5M NaOH for cleaning-in-place (CIP)
- Permits significant cost savings and extension of cycles in regular production
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|4° to 30°C|
|≈35mg Human lgG/mL at 2.4 min. residence time, ≈35mg Human lgG/mL at 2.4 min. residence time|
|HiTrap MabSelect SuRe, 5 x 1 mL|
|For fast purification of monoclonal antibodies from large sample volumes, affinity purification|
|HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess resin for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).|
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