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Abnova™ Histone H3 (Total) Quantification Kit (Colorimetric)

Histone H3 (Total) Quantification Kit (Colorimetric) is designed for quantifying levels of histone H3 proteins independent of its modified state and can also be used for normalizing the modified histone H3 content of samples when run in parallel with other histone modification quantification kit series.

Manufacturer:  Abnova™ KA0624

Catalog No. 89-027-185


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Includes:
  • C1 (10X wash buffer) 20mL
  • C2 (antibody buffer) 12mL
  • C3 (detection antibody, 1mg/ml) 10µL
  • C4 (color developer) 10mL
  • C5 (stop solution) 6mL
  • Standard control (100µg/ml) 20µL
  • 8 well sample strips (with frame) 9
  • 8 well standard control strips 3

Description

Description

  • Quick and efficient procedure which can be finished within 2.5 hours
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography
  • Specifically captures histone H3 with the detection limit as low as 10 ng/well and detection range from 20 ng-1 µg/well of histone extracts
  • The unmodified histone H3 control is conveniently included for quantification of the amount of total histone H3
  • Strip microplate format makes the assay flexible, manual or high throughput
  • Simple, reliable, and consistent assay conditions
  • Upon receipt store C3 and Standard control at -20°C; store all other components at 4°C away from light

Specifications

Specifications

Histone H3 (Total) Quantification Kit (Colorimetric) is designed for quantifying levels of histone H3 proteins independent of its modified state and can also be used for normalizing the modified histone H3 content of samples when run in parallel with other histone modification quantification kit series.
Histo ne H3 (to tal) quantification Kit
Colorimetric
Quantification
Store C3 and Standard control at -20°C. Store all other components at 4°C away from light.
96 Assays
Documents
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