Invitrogen AccuPrime Pfx DNA Polymerase
Provides higher fidelity than Pfu DNA polymerase
Manufacturer: Invitrogen 12344024
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.
- Achieve greater than 26 times higher fidelity than Taq DNA polymerase
- Amplify fragments up to 12 kb
- Minimize PCR optimization
- Built-in hot start
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research
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|• 1 x 100 μL AccuPrime Pfx DNA Polymerase (2.5 U/µl)
• 1 x 1 mL 10X AccuPrime Pfx Reaction Mix
• 1 x 1 mL 50 mM MgSO4
Store at -20°C.
|Built-In Hot Start|
|AccuPrime™ Pfx DNA Polymerase|
|12 kb or less|
|3' - 5'|
|Approved for shipment on Wet or Dry Ice|
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For Research Use Only. Not for use in diagnostic procedures.