Invitrogen™ ACE Mouse ELISA Kit

Sandwich ELISA Kit

Catalog Number	Mfr. No.	Quantity
EMACE	Invitrogen™ EMACE	96 Tests
EMACEX5	Invitrogen™ EMACEX5	5 x 96 Tests
EMACEX10	Invitrogen™ EMACEX10	10 x 96 Tests

Description

The Mouse Angiotensin Converting Enzyme (Ms ACE) ELISA quantitates Ms ACE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms ACE. Principle of the method The Mouse ACE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.

Specifications

• Accession Number: P09470
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AW208573,CD143
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 0.12 ng/mL
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 11421
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Cardiovascular Research
• Synonym: RP23-100A9.1, AW208573, CD143
• Test Time: 1 hr. 20 min.