BioVision Inc Aconitase Activity Colorimetric Assay Kit: Aconitase Activity Assay Kit: High-Throughput Colorimetric Assay for Measuring Aconitase Activity in various Biological Samples such as Tissues, Cells etc. Simple, Direct & Sensitive. 100 Assays.
Manufacturer: BioVision Inc K716100
Aconitase (aconitate hydratase; EC 184.108.40.206) is an iron-sulfur protein containing an [Fe₄S₄]²ᶧ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm. BioVision charges an Ice pack fee for each order.
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