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Actin (muscle) Mouse anti-Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Non-human primate, Porcine, Rat, Clone: HHF35, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 14649680

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Catalog No. 50-245-578

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Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas. The HHF35 antibody recognizes human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine and non-human primate actin. Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections and western blotting. Applications Tested: This HHF35 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (cat. 00-4955)) and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.


Actin (muscle)
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P62739, P68138, Q3ZC07, P62736, P68032, P68133, P62737, P68033, P68134, P68137, P62738, P68035, P68136
actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha cardiac muscle 1; actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; alpha actin 1; alpha actin 2; alpha skeletal muscle; alpha skeletal muscle actin; alpha-actin cardiac; alpha-actin-1; alpha-actin-2; alpha-cardiac actin; alpha-sma; alphac-actin; cell growth-inhibiting gene 46 protein; nemaline myopathy type 3; skeletal alpha actin; smooth muscle alpha-actin
Affinity chromatography
Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Non-human primate, Porcine, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
ACTA1, Acta2, ACTC1
IgG1, kappa
25 μg
4° C
100057678, 100062175, 100065454, 100152267, 100154254, 100716933, 100722917, 100731953, 11459, 11464, 11475, 281592, 29275, 29437, 477587, 478250, 488984, 515610, 533219, 58, 59, 70, 733615, 81633
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