BioVision Inc ADP Colorimetric/Fluorometric Assay Kit: ADP Colorimetric/Fluorometric Assay Kit: Robust & Convenient Colorimetric & Fluorometric Assay to Measure ADP Levels within an Hour. 100 Assays.
Manufacturer: BioVision Inc K355100
ADP Colorimetric/Fluorometric Assay Kit: ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. BioVision's newly designed ADP Assay Kit provides a convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (?max = 570 nm) or fluorometric method (Ex/Em 535/587 nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1 ¿M ADP in biological samples.
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