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Vector LaboratoriesSupplier Diversity Partner Succinylated Wheat Germ Agglutinin (WGA) Agarose Bound 5 ml DFS Item

Manufacturer:  Vector LaboratoriesSupplier Diversity Partner AL1023S5

Wheat germ agglutinin (WGA) can bind to oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues. Succinylated WGA does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Agarose Bound Succinylated WGA is prepared using affinity-purified lectins and heat stable, cross-linked 4% agarose beads. The attachment of the lectins through a hydrophilic spacer arm to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins. The recommended inhibiting/eluting sugar is chitin hydrolysate or 500 mM N-acetylglucosamine. Salt and/or acid elution may also be required.

Catalog No. NC1470947


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