AHR Monoclonal Antibody (4MEJJ), eFluor™ 660, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 50592580

Catalog No. 50-112-4409

Description

Description: This 4MEJJ monoclonal antibody recognizes mouse aryl hydrocarbon receptor (AHR). The AHR is a ligand-activated transcription factor that mediates the toxic effects of a diverse group of environmental contamits, most notably aryl hydrocarbons such as polychlorinated biphenyls (PCB) and tetrachlorodibenzo-p-dioxin (TCDD). The AHR has also been shown to bind to a number of naturally occurring compounds found in fruits and vegetables as well as compounds generated through normal cellular metabolism. AHR is localized in the cytoplasm in a complex that includes HSP90, p23, and XAP2/AIP/ARA9. Upon ligand-binding, AHR translocates to the nucleus and binds with aryl hydrocarbon receptor nuclear translocator (ARNT), and this complex binds to the consensus DNA sequence, GCGTG, found in the promoter/enhancer regions of many genes such as CYP1A1. The AHR is expressed in many cell types, with highest expression levels found in liver. The AHR has been shown to play a role in the regulation/differentiation of Treg and Th17 cells. The 4MEJJ monoclonal antibody has been found to recognize the b-1, b-2 and d alleles of mouse AHR but does not crossreact with human AHR. Applications Reported: This 4MEJJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

AHR Monoclonal antibody specifically detects AHR in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: AHR
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P30561
• Gene Symbols: AHR
• Immunogen: E. coli derived, His-tagged AHR (aa 443-805)
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 4MEJJ
• Conjugate: eFluor 660
• Gene: AHR
• Gene Alias: Ah; Ah receptor; Ahh; Ahr; Ahre; AH-receptor; aromatic hydrocarbon receptor; aryl hydrocarbon receptor; aryl-hydrocarbon receptor; bHLHe76; Class E basic helix-loop-helix protein 76; dioxin receptor; In
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11622
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

For Research Use Only.