AIRE Monoclonal Antibody (TM-724), eFluor™ 570, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 41953482

Catalog No. 50-112-3644

Description

Description: This TM-724 monoclonal antibody reacts with human Autoimmune Regulator (AIRE). This 58 kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). AIRE mRNA in the periphery has been observed in the lymph node, fetal liver, and intestines. Furthermore, expression has been observed in dendritic cells, epithelial cells and PBMCs. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation. As a result of alternative splicing, three isoforms have been reported. The conditions autoimmune-polyendocrinopathy-candiasis-ectodermal-dystrophy (APECED) and autoimmune polyglandular syndrome type 1 (APS-1) arise from mutations in the human AIRE gene. The TM-724 antibody is predicted to recognize all three isoforms of human AIRE (AIRE-1, AIRE-2, AIRE-3). Applications Reported: This TM-724 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This TM-724 antibody has been tested by immunohistochemistry on FFPE human thymus at less than or equal to 10 μg/mL using low pH antigen retrieval.

AIRE Monoclonal antibody specifically detects AIRE in Human samples. It is validated for Immunocytochemistry, Immunohistochemistry (Paraffin).

Specifications

• Antigen: AIRE
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O43918
• Gene Symbols: Aire
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 326
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin)
• Clone: TM-724
• Conjugate: eFluor 570
• Gene: Aire
• Gene Alias: Aire; AIRE protein; AIRE1; APECED; APECED protein; APECED protein homolog; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog; Autoimmune regulator; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy); autoimmune regulator AIRE1a; LOW QUALITY PROTEIN: autoimmune regulator; OTTHUMP00000109529; PGA1
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.