Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Molecular Probes™ AlamarBlue™ Cell Viability Reagent Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

AlamarBlue™ Cell Viability Reagent

$245.00 - $492.00

Specifications

Color Blue
Detection Method Colorimetric, fluorescent
Excitation Wavelength Range 530 to 560nm
For Use With (Application) For cell counting, viability, and cryopreservation, cell viability, proliferation and function
For Use With (Equipment) For microplate reader, spectrophotometer
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
DAL1025 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. View Documents Molecular Probes™
DAL1025
25mL Each for $245.00
Add to cart
 
DAL1100 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only. View Documents Molecular Probes™
DAL1100
100mL Each for $492.00
Add to cart
 
Description

Description

alamarBlue reagent is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.

Applications:
  • Cell proliferation determinations—alamarBlue reagent is water soluble, stable in culture, and non-toxic
  • Cell viability assays, including cytotoxicity—metabolic activity and dye generation changes in proportion to altered viability and cell damage
  • Cytokine assays—measure cytokine-induced proliferation, recover and expand cells at the end of the study if desired
alamarBlue reagent as a cell viability assay reagent
The alamarBlue assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator. Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal. The dye itself has low toxicity, and is generally safe for cells.

A safe, convenient, and versatile assay
alamarBlue reagent is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells. Since alamarBlue reagent is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study. alamarBlue reagent is water-soluble, saving time otherwise spend on washing and extraction steps. The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium. The protocol is readily amenable to automation and high-throughput assays. The ability to detect the alamarBlue indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy.

Experimental detection of alamarBlue reagent
  • Fluorometry: by excitation at 530–560 nm and emission at 590 nm
  • Spectrophotometry: by absorbance measurements at 570 nm and 600 nm

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

Specifications

Blue
530 to 560nm
For microplate reader, spectrophotometer
2 to 8°C
Colorimetric, fluorescent
For cell counting, viability, and cryopreservation, cell viability, proliferation and function
384-well plate, 96-well plate, Cuvettes
AlamarBlue™ Cell Viability Reagent

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit