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Invitrogen™ Albumin Human ELISA Kit
GSA_VA

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHALB

Encompass

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Catalog No. EHALB


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Description

Description

The Human Albumin (Hu ALB) ELISA quantitates Hu ALB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ALB. Principle of the method The Human ALB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Albumin is a soluble, monomeric protein with a molecular weight of approximately 65-67 kDa that comprises about one-half of the blood serum protein. Human serum albumin (HAS) is the most abundant protein in human blood plasma, and has a serum half-life of approximately 20 days. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular, unglycosylated serum protein synthesized in the liver as pre-pro-albumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough `produce the secreted albumin. Serum albumin has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. The main function of albumin is the regulation of the colloidal osmotic pressure of blood.

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Specifications

Specifications

ELISA Kit
This ELISA pair antibody detects human Albumin. Other species not determined.
Solid-phase sandwich ELISA
Biotin
P02768
60pg/mL to 12,000pg/mL
213
RUO
2°C to 8°C
4 hr. 45 min.
Other Research
<12%
96 Tests
Plasma, Serum, Supernatant
HSA,PRO0883,PRO0903,PRO1341
Colorimetric Microplate Reader
96 assays
Other Proteins
HRP
4.915-1200 ng/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
GIG20, ANALBA, FDAH, PRO0883, PRO0903, PRO1341
<10%
Human
6 pg/mL
SDS
Documents
Product Certifications
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