Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

TCR alpha/beta Mouse anti-Human, PE, Clone: WT31, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12995542

 View more versions of this product

Catalog No. 50-113-17


Add to cart

Description

Description

Description: This WT31 monoclonal antibody recognizes the human alpha beta T cell receptor (TCR) complexed with the CD3 epsilon chain. Composed of a 44-kDa alpha and a 37-kDa beta chain, TCR specificity is typically determined by V alpha, J alpha, V beta, D beta, and J beta gene rearrangement. The TCR is often associated with the CD3 complex, which is composed of at least five polypeptides. The TCR/CD3 complex recognizes and binds to major histocompatibility complex-bound antigen presented by antigen presenting cells such as B cells, dendritic cells, and macrophages. These events lead to T cell activation, resulting in cellular signaling and cytokine production. The WT31 monoclonal antibody has been reported to induce proliferation of resting T cells, as well as inhibit cytotoxic T cell activity. In addition, some antibodies against CD3 partially inhibit alpha beta TCR antibody staining using WT31; clone SK7 (anti-human CD3) is recommended for use in two-color experiments. Applications Reported: This WT31 antibody has been reported for use in flow cytometric analysis. Applications Tested: This WT31 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and associate with the CD3 subunits in the endoplasmic reticulum to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.
Specifications

Specifications

TCR alpha/beta
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P04435, P01848
Tcrb, Tcra, FLJ22602, MGC117436, MGC22624, MGC23964, MGC71411, TCRA, TCRD, TRA, T-cell antigen receptor, alpha polypeptide, T-cell receptor, TCR beta
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
WT31
PE
TCRB
Liquid
TCRA, TCRB
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
6955, 6957
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only.