alpha-Fetoprotein/AFP Mouse anti-Human, Clone: MBS-12, Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP248015A	Novus Biologicals NBP2480150.1MG	0.1 mg
NBP248015B	Novus Biologicals NBP2480150.2MG	0.2 mg

Description

alpha-Fetoprotein/AFP Monoclonal specifically detects alpha-Fetoprotein/AFP in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: alpha-Fetoprotein/AFP
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cancer Stem Cells, GPCR, Stem Cell Markers, Tumor Biomarkers
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 174
• Immunogen: Recombinant full-length human Alpha fetoprotein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 70 kDa

• Clone: MBS-12
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: Alpha-1-fetoprotein, Alpha-fetoglobulin, alpha-fetoprotein, FETA, HP, HPAFP
• Gene Symbols: AFP
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. The yolk sac and the liver produce AFP during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spinal bifida and anencephaly.