alpha Tubulin, clone: DM1A, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 14450280
Tubulin is a heterodimer consisting of alpha and beta-tubulin subunits. It is nearly ubiquitous among eukaryotic cells. The alpha and beta tubulins, which are each about 55kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules. This intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosisDescription: The monoclonal antibody DM1a recognizes the 50kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430).Applications Reported: This DM1A antibody has been reported for use in immunocytochemistry, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This DM1A antibody has been tested by western blotting under reducing conditions (at 2ug/ml) or by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2µm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Q71U36, P68369, P02550, P68370|
|FLJ30169, H2 ALPHA, Testis specific alpha tubulin, TUBA1, Tubulin alpha|
|Canine, Human, Mouse, Non-human primate, Porcine, Rat|
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot|
|100135051, 22142, 64158, 7846|
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