|10mL Resuspension Buffer; 1mL Lysis Enhancer; 2 x 1.25mL DNase I, Amplification Grade (1U/μL); 800μL 10X DNase I Buffer; 2 x 1mL 25mM EDTA; 500μL SuperScript III RT/Platinum Taq Mix; 12.5mL 2X Reaction Mix; 1mL 50mM MgSO4; 12.5mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL); 500μL ROX Reference Dye|
|7500 Fast System, 7500 System, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, Corbett RotorGene, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000|
|ROX Reference Dye (store at -20°C in the dark)|
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