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Invitrogen™ MAXIscript™ SP6 Kit with Manual

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

$211.00 - $414.00

Specifications

Content And Storage SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Final Product Type synthetic mRNA, mRNA
Format Kit
Sample Type mRNA, synthetic mRNA
For Use With (Application) In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
View More Specs

Includes: SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, Nuclease-free Water

Products 4
Catalog Number Mfr. No. Product Type Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Product Type Quantity Price Quantity & Availability  
AM1310M
Encompass
View Documents
Invitrogen™
AM1310M
SP6 In Vitro Transcription kit 100 Reactions
Each for $414.00
Add to cart
 
AM1310
Encompass
View Documents
Invitrogen™
AM1310
SP6 In Vitro Transcription kit 100 Reactions
Each for $399.00
Add to cart
 
AM1308
Encompass
View Documents
Invitrogen™
AM1308
SP6 In Vitro Transcription kit 30 reactions
Each for $211.00
Add to cart
 
AM1308M
Encompass
View Documents
Invitrogen™
AM1308M
SP6 In Vitro Transcription kit 30 reactions
Each for $214.00
Add to cart
 
Description

Description

Ambion MAXIscript SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions.

  • High yields of high specific activity, full-length RNA probes in just 10 minutes (RNA Yield: 2-6μg)
  • Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
  • Includes TURBO DNase
    Contain quality-tested DNase/RNase-free reagents
  • Synthesizes RNA probes with specific activities reaching 1 x 109 cpm/μg in just 10 minutes
  • Efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them perfect companion to ribonuclease protection assays
  • For synthesis of nonisotopically labeled probes
  • Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into reaction
  • For synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb

Specifications

Specifications

SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Kit
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
SP6 In Vitro Transcription kit
2-6 µg
synthetic mRNA, mRNA
mRNA, synthetic mRNA
Ambion™, MAXIscript™
SP6
PCR Products, Linearized DNA plasmid
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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