Invitrogen™ PureLink™ Pro 96 total RNA Purification Kit

Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

Supplier:  Invitrogen™ 12173011A

Catalog No. 12-173-011A

Description

Isolate high yields of total RNA with low genomic DNA contamination from bacteria, yeast, plant, or mammalian samples.

The one-hour procedure requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water; ideal for use in a variety of applications, including RT-PCR, qPCR, northern blotting, and nuclease protection assays.

Improved plate design
The PureLink™ Pro 96 total RNA Purification Kit provides optimized semi-skirted 96-well plates for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at 2250 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Specifications

• Content And Storage: • 150 mL PureLink Pro 96 Lysis Buffer; store at room temperature
  • 250 mL PureLink Pro 96 Wash Buffer I; room temperature
  • 250 mL PureLink Pro 96 Wash Buffer II; room temperature
  • 75 mL PureLink Pro 96 RNase-free Water; room temperature
  • 4 PureLink 96-well Total RNA Filter Plates; room temperature
  • 4 PureLink Pro 96 Elution Plates; room temperature
• Purification Time: 25 min. (After sample homogenization)
• Sample Type: Bacteria, Cells, Plant, Tissue, Yeast
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Cells: varies
  Plant: ≤150 mg
  Tissue: ≤100 mg
  Yeast: ≤1.8 mL

• Isolation Technology: Silica Plate
• Elution Volume: 45–170 μL
• Final Product Type: Total RNA
• For Use With (Equipment): Biomek FX, Robotic Liquid Handling Systems, Eppendorf epMotion TECAN Freedom Evo, TECAN Genesis
• Quantity: 4 Plates
• Shipping Condition: Room Temperature
• Yield: Up to 33 μg

For Research Use Only. Not for use in diagnostic procedures.