Invitrogen™ RiboMinus™ Transcriptome Isolation Kit, human/mouse  

Removes 95 to 98% of rRNA

Manufacturer:  Invitrogen™ K155002

Catalog No. K155002

Includes: The RiboMinus Isolation Kits include RiboMinus Magnetic Beads, RiboMinus human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.

Description

RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5µ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95 to 98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA. This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods.
Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis, library construction, and qRT-PCR.

• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios

Not high-throughput compatible (Manual)

DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Extraction, RNA Isolation for Expression Microarrays, Transcriptome RNA Isolation

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Specifications

• Final Product Type: Transcriptome RNA
• Purification Target: Transcriptome RNA
• Format: Magnetic Bead
• No. of Reactions: 6 Preps
• Yield: 1 µg
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Starting Material Amount: Up to 10 µg total RNA
• Product Line: RiboMinus™

• For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
• Sample Type: Total RNA
• Isolation Technology: Magnetic Bead
• Quantity: 1
• Content And Storage: The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer. The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water. Store the isolation modules at +4°C and the concentration module at room temperature. All reagents are guaranteed stable for 6 months when properly stored.
• Purification Time: 1.25 hrs
• System Type: RiboMinus

For Research Use Only. Not for use in diagnostic procedures.