Ambion™ TaqMan™ Gene Expression Cells-to-CT™ Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Perform expression analysis directly from cultured cells without RNA purification

Manufacturer: Invitrogen 4399002

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Catalog No. 43-990-02

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    Specifications

    Detection Method Primer-Probe Detection
    Dye Type ROX (Pre-mixed)
    For Use With (Application) Drug Discovery and Development, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, PCR-Based Drug Discovery Assays, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples, Target and Lead Identification and Validation
    Includes Contains: Stop Solution: 200μL tube; DNase I: 22μL tube; 20X RT Enzyme Mix: 110μL tube; Lysis Solution: 2.2mL tube; 2X RT Buffer: 2.2mL tube; TaqMan Gene Expression Master Mix: 1mL tube
    No. of Tests 40.0
    Storage Requirements Store Stop Solution, DNase I, and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X RT Buffer, and TaqMan Gene Expression Master Mix at 4°C.

    Kkit saves time and offers simple workflow suitable for few samples or can be easily incorporated into automated, high-throughput applications.

    • Complete: optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, buffer, and new TAqMan Gene Expression Master Mix
    • Fast: 7-minute sample prep, including DNase treatment, at room temperature
    • Easy: lyse samples in a tube or directly in culture plates
    • Robust: perform gene expression analysis on 10-100,000 cells per sample; results equivalent to those from purified RNA
    • Efficient: contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples
    • Fewer resources used, less hazardous, less waste

    Drug Discovery and Development, Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, PCR-Based Drug Discovery Assays, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples, Target and Lead Identification and Validation

  • Description & Specifications

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