Ambion WT Expression Kit, High Throughput
Generates amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix GeneChip WT Terminal Labeling Kit
Manufacturer: Invitrogen 4440536
|Kit, WT Expression|
|150μL First-Strand Enzyme Mix, 600μL First-Strand Buffer Mix, 650μL Second-Strand Enzyme Mix, 1.45mL Second-Strand Buffer Mix, 850μL IVT Enzyme Mix, 1.4mL IVT Buffer Mix, 10μL Control RNA 1mg/mL, 1.45mL 2nd-Cycle Buffer Mix, 300μL Random Primers, 1mL 2nd-Cycle Enzyme Mix, 300μL RNase H, 12.5mL Nucleic Acid Binding Buffer Concentrate, 2.5mL Nucleic Acid Binding Beads, 50mL Nucleic Acid Wash Solution Concentrate, 22mL Elution Solution|
|Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Amplification|
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