Ambion WT Expression Kit, High Throughput
Generates amplified sense-strand cDNA ready for fragmentation and labeling using the Affymetrix GeneChip WT Terminal Labeling Kit
Manufacturer: Invitrogen 4440537
|Kit, WT Expression|
|50μL First-Strand Enzyme Mix, 160μL First-Strand Buffer Mix, 175μL Second-Strand Enzyme Mix, 400μL Second-Strand Buffer Mix, 210μL IVT Enzyme Mix, 750μL IVT Buffer Mix, 10μL Control RNA 1mg/mL, 425μL 2nd-Cycle Buffer Mix, 100μL Random Primers, 275μL 2nd-Cycle Enzyme Mix, 100μL RNase H, 3.5mL Nucleic Acid Binding Buffer Concentrate, 700μL Nucleic Acid Binding Beads, 15mL Nucleic Acid Wash Solution Concentrate, 6mL Elution Solution|
|Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Amplification|
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