AMPK beta-1 Recombinant Polyclonal Antibody (22HCLC), Invitrogen™

Rabbit Recombinant Polyclonal Antibody

Supplier:  Thermo Scientific 711320

Catalog No. PI711320

Description

This antibody is predicted to react with Monkey, Cat and Bovine Recombit rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombit monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombit antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombit rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

AMPK beta 1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Specifications

• Antigen: AMPK beta-1
• Classification: Recombinant Polyclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: Q9Y478, P80386
• Gene Symbols: PRKAB1
• Immunogen: Peptides corresponding to Human PRKAB1 (aa 27-36, 166-180)
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Clone: 22HCLC
• Conjugate: Unconjugated
• Gene: PRKAB1
• Gene Alias: 5′-AMP-activated protein kinase subunit beta-1
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 5564, 83803
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Research Use Only