BioVision Inc Annexin V-Cy3 Apoptosis Kit Plus: Annexin V-Cy3 Apoptosis Detection Kit Plus: Simple One Step Assay to Detect Apoptotic, Necrotic & live cells within 10 min. No need to fix the cells. Detection: Flow Cytometry or fluorescence microscopy
Manufacturer: BioVision Inc K20225
Annexin V-Cy3 Apoptosis Kit Plus: The assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The Kit Plus includes annexin V-Cy3, SYTOX green dye, and binding buffer. After staining a cell population with annexin V-Cy3 and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm). BioVision charges an Ice pack fee on order.
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